PASTA: versatile tyramide-oligonucleotide amplification for multimodal spatial biology

Hendrik A Michel, Paige McCallum, Wenrui Wu, Huaying Qiu, Jia Le Lee, Shuli Luo, Chi Ngai Chan, Johanna Schaffenrath, Yao Yu Yeo, Stephanie Pei Tung Yiu, Yang Wang, Lindsay Parmelee, Hongbo Wang, Tania Pannellini, Melinda Burgess, Nourhan El Ahmar, Zoe Xiaozhu Zhang, Colm Keane, Tony Kiat Hon Lim, Sabina Signoretti, Sonia Victoria Del Rincon, Bo Zhao, David R McIlwain, Yunhao Bai, Fei Chen, Roberto Chiarle, Sizun Jiang

Abstract

Spatial proteomics is limited by detection sensitivity, multiplexing and multimodal integration, leaving a gap between discovery and clinical assays. Here we present protein and nucleic acid serial tyramide amplification (PASTA), using horseradish peroxidase-mediated oligonucleotide deposition and cyclical imaging for high-plex, multimodal spatial profiling. Compatible with conjugated antibodies and in situ hybridization, PASTA enables simultaneous protein and RNA codetection from formalin-fixed, paraffin-embedded samples, providing a cost-effective bridge from discovery to clinical validation.

<<< GO BACK

GO TO PAPER >>>